Cas12a(又称cpf1)是一类由单个crrna介导的核酸内切酶,能特异性识别并剪切带pam(protospacer adjacent motif,即原间隔区邻近基序)的双链dna(dsdna)靶标。 不同来源的cas12a蛋白识别的pam序列可能有所不同。 cas12a家族都识别富含t的pam序列。
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